概述

  • 产品名称Anti-Noradrenaline transporter抗体
    参阅全部 Noradrenaline transporter 一抗
  • 描述
    兔多克隆抗体to Noradrenaline transporter
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 396 - 445 (STLSGSTFWA VVFFVMLLAL GLDSSMGGME AVITGLADDF QVLKRHRKLF) of Human Noradrenaline transporter (NP_001034)

  • 阳性对照
    • Human fetal heart lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 疾病相关Orthostatic intolerance
  • 序列相似性Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NAT1 antibody
    • NET antibody
    • NET1 antibody
    • Neurotransmitter transporter antibody
    • Norepinephrine transporter antibody
    • SC6A2_HUMAN antibody
    • Slc6a2 antibody
    • SLC6A5 antibody
    • Sodium dependent noradrenaline transporter antibody
    • Sodium-dependent noradrenaline transporter antibody
    • Solute carrier family 6 (neurotransmitter transporter norepinephrine) member 5 antibody
    • Solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 antibody
    • Solute carrier family 6 member 2 antibody
    • Solute carrier family 6 member 5 antibody
    see all

Anti-Noradrenaline transporter antibody 图像



  • Predicted band size : 70 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling Noradrenaline transpoter with ab84057 at 1.0µg/ml.


  • Predicted band size : 70 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling Noradrenaline transpoter with ab84057 at 1.0µg/ml.
  • Anti-Noradrenaline transporter antibody (ab84057) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • ICC/IF image of ab84057 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84057, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Noradrenaline transporter antibody (ab84057)参考文献

This product has been referenced in:
  • Bayles R  et al. Epigenetic modification of the norepinephrine transporter gene in postural tachycardia syndrome. Arterioscler Thromb Vasc Biol 32:1910-6 (2012). Read more (PubMed: 22723437) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab84057.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"