Customer reviews and FAQs

概述

  • 产品名称
    Anti-Noradrenaline抗体
  • 描述
    兔多克隆抗体to Noradrenaline
  • 特异性
    ab8887 targets conjugated noradrenaline only it does not recognize free noradrenaline.
  • 经测试应用
    适用于: ICC, IHC-Fr, IHC-Pmore details
  • 免疫原

    Chemical/ Small Molecule corresponding to Noradrenaline conjugated to Bovine Serum Albumin (BSA).

性能

应用

Our Abpromise guarantee covers the use of ab8887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
IHC-Fr
IHC-P
  • 应用说明
    ELISA: Use at an assay dependent concentration.
    ICC: Use at an assay dependent concentration.
    IHC-P: Use at an assay dependent dilution (PMID 18457899).
    IHC-Fr: Use at an assay dependent concentration.

    The recommended dilution can be between 1:2,000-1:5,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 相关性
      Noradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.
    • 细胞定位
      Secreted
    • 别名
      • Arterenol antibody
      • NA antibody
      • Norepinephrine antibody

    Anti-Noradrenaline antibody 图像

    • Immunocytochemistry/ Immunofluorescence - Noradrenaline (conjugated) antibody (ab8887)
      Immunocytochemistry/ Immunofluorescence - Noradrenaline (conjugated) antibody (ab8887)Image from Johnson KB et al., Front Physiol. 2010 Oct 4;1:131. Fig 4.; doi: 10.3389/fphys.2010.00131; 04 October 2010, Front. Physio. 1:131.
      ab8887 staining Noradrenaline (red) in rat smooth muscle cells from the aorta.

      Cells were transfected with conjugated Noradrenaline before being fixed and permeabilized with 0.1% Triton X-100 for 20 minutes. Samples were incubated with primary antibody at 1/500 dilution. A fluorescence-conjugated anti-rabbit IgG was used as the secondary antibody.

    Anti-Noradrenaline antibody (ab8887)参考文献

    This product has been referenced in:
    • Kondo Y  et al. Expression and Role of the BDNF Receptor-TrkB in Rat Adrenal Gland under Acute Immobilization Stress. Acta Histochem Cytochem 43:139-47 (2010). ICC/IF . Read more (PubMed: 21245980) »
    • Johnson KB  et al. Modification of proteins by norepinephrine is important for vascular contraction. Front Physiol 1:131 (2010). WB, ICC/IF, IHC-P . Read more (PubMed: 21423373) »

    See all 4 Publications for this product

    发表研究结果有使用 ab8887？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab8887 的 abreview 提交 ab8887 的问题

    8 Abreviews or Q&A

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    Votes: Highest First

    Inquiry: Dear, Can you send me the MSDS sheet for the product ab8887 'Noradrenaline (conjugated) antibody? Thank you very much, With kind regards,

    Thank you for your enquiry and interest in our products.

    The storage buffer of this antibody only contains Glycerol which is nonhazardous, there is no MSDS for ab8887. A general SDS document can be downloaded from the on-line datasheet.
    ...

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    thank you so much for your reply. I will be perfusing tissue
    shortly (next week). My plans were to perfuse with 4% PFA, but I'm hoping you
    might be able to tell me if I need to perfuse with a solution that includes
    glutaraldehyd...

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    Thank you very much for your reply.

    For ab6454, it looks like the antibody was tested with glutaraldehyde-perfused tissue sections. There is a link to "Immunohisto and cytochemistry" protocol at the very bottom of the datasheet- Read More

    Inquiry: Noradrenaline (conjugated) antibody (ab8887) Hi abcam- I'm interested in your anti-NA antibodies and I see that you have one that is congugated (Ab8887) and one that is not (AB6465). I am uncertain that I understand the difference between the ...

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    Thank you very much for contacting us with your enquiry.

    I apologize for the confusion regarding these products. Both ab8887 and ab6454 are antibodies that target conjugated noradrenaline, instead of free noradrenaline. As a small molecule, N...

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    Hello our lab is organizing our MSDS sheets and I was having trouble finding sheets for some of your products, specifically ab8883, ab8887, and ab8885. Could you please direct me to this information on your website or send me the MSDS sheets for these ...

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    Thank you for contacting Abcam.


    Attached please find the MSDS you requested.


    Please do not hesitate to contact me if you have any additional questions.

    What is the concentration of this antibody?

    Thank you for contacting Abcam regarding ab8887.

    I have confirmed with the laboratory that unfortunately, there is no concentration information available for this antibody.

    I apologize for any inconvenience this may cause. Please ...

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    On FAQ, this antibody has been used on formalin-fixed tissue sections but the reply from Abcam was that this antibody has not been tested on formalin-fixed tissue. This is in contrast to your datasheet which states that this antibody can be used on IHC...

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    Thank you for your enquiry. The FAQ you noticed is from Thursday 25-November-2004 at which time the antibody had not been tested in IHC-P. We since have data from a publication that came to our attention in Sept 2008. PMID 18457899. Thi...

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    In our lab we use a phenol-ethanol supernatant obtained after precipitation of DNA with ethanol, we wonder if we could use this supernatant for the ELISA with acetilcholine and noradrenaline antibodies. The protocols displayed in the internet page, ...

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    Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested t...

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    Thank you for your questions. I would like to answer them one by one. > 1. Please describe the problem (high background, no staining etc). answer: no staining, no positive results. > 2. On what material are you testing the antibody in IHC? ans...

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    Thank you for your patience and forwarding your detailed protocol on-line. We are very sorry to hear that you are having some problem with this antibody. Looking through your email, I can understand that you have fixed the tissue sections in parafor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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