- 数据表
- 具体的参考文献 (5)
- 实验方案
概述
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产品名称Anti-Noradrenaline抗体
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描述兔多克隆抗体to Noradrenaline
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宿主Rabbit
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特异性ab8887 targets conjugated noradrenaline only it does not recognize free noradrenaline.
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经测试应用适用于: ICC, IHC-Fr, IHC-Pmore details
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免疫原
Chemical/ Small Molecule corresponding to Noradrenaline conjugated to Bovine Serum Albumin (BSA).
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: None
Constituents: 50% Glycerol, ddH20 -
Concentration information loading... -
纯度IgG fraction
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab8887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | ||
| IHC-Fr | ||
| IHC-P |
应用说明
ELISA: Use at an assay dependent concentration.
ICC: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution (PMID 18457899).
IHC-Fr: Use at an assay dependent concentration.
The recommended dilution can be between 1:2,000-1:5,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution (PMID 18457899).
IHC-Fr: Use at an assay dependent concentration.
The recommended dilution can be between 1:2,000-1:5,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性Noradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.
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细胞定位Secreted
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别名
- Arterenol antibody
- NA antibody
- Norepinephrine antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline antibody (ab8887)Image from Johnson KB et al., Front Physiol. 2010 Oct 4;1:131. Fig 4.; doi: 10.3389/fphys.2010.00131; 04 October 2010, Front. Physio. 1:131.ab8887 staining Noradrenaline (red) in rat smooth muscle cells from the aorta.
Cells were transfected with conjugated Noradrenaline before being fixed and permeabilized with 0.1% Triton X-100 for 20 minutes. Samples were incubated with primary antibody at 1/500 dilution. A fluorescence-conjugated anti-rabbit IgG was used as the secondary antibody.
文献
This product has been referenced in:
- Yadirgi G et al. Immuno-detection of cleaved SNAP-25 from differentiated mouse embryonic stem cells provides a sensitive assay for determination of botulinum A toxin and antitoxin potency. J Immunol Methods 451:90-99 (2017). ICC/IF . Read more (PubMed: 28943257) »
- Kondo Y et al. Expression and Role of the BDNF Receptor-TrkB in Rat Adrenal Gland under Acute Immobilization Stress. Acta Histochem Cytochem 43:139-47 (2010). ICC/IF . Read more (PubMed: 21245980) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"