Anti-Non Neuronal Enolase抗体(ab49343)


  • 产品名称Anti-Non Neuronal Enolase抗体
    参阅全部 Non Neuronal Enolase 一抗
  • 描述
    兔多克隆抗体to Non Neuronal Enolase
  • 特异性This antibody is specific for Non Neuronal Enolase.
  • 经测试应用适用于: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Guinea pig, Dog, Pig, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region of Human Non Neuronal Enolase, derived from within the following sequence (aa 246-295): VAASEFFRSG KYDLDFKSPD DPSRYISPDQ LADLYKSFIK DYPVVSIEDP.

  • 阳性对照
    • Fetal Intestine lysate and human kidney tissue


Our Abpromise guarantee covers the use of ab49343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 0.03 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr 1/250.
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • 组织特异性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 序列相似性Belongs to the enolase family.
  • 发展阶段During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 翻译后修饰ISGylated.
  • 细胞定位Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2-phospho-D-glycerate hydro-lyase antibody
    • alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • bh3 interacting domain death agonist antibody
    • c-myc promoter-binding protein 1 antibody
    • C-myc promoter-binding protein antibody
    • c-myc promoter-binding protein-1 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • enolase 1 (alpha) antibody
    • Enolase 1 antibody
    • HAP 47 antibody
    • HAP47 antibody
    • MBP-1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter-binding protein 1 antibody
    • NNE antibody
    • Non-neural enolase antibody
    • OTTHUMP00000001706 antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • tau-crystallin antibody
    see all

Anti-Non Neuronal Enolase antibody 图像

  • Anti-Non Neuronal Enolase antibody (ab49343) at 0.03 µg/ml + Fetal intestine lysate 10µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human kidney tissue labelling Non Neuronal Enolase with ab49343 at 4-8µg/ml. Arrows indicate positively labellled epithelial cells of collecting tubules. Magnification: 400X.

  • ab49343 staining mouse embryonic brain slice tissue sections by IHC-Fr.  Tissue sections were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X in 0.1M PBS.  The sample was blocked with 5% serum for 2 hours at 4°C prior to incubation with the primary antibody, diluted 1/20 in 0.1M PBS for 24 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-rabbit IgG antibody was used as the secondary.

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  • ICC/IF image of ab49343 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49343, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Non Neuronal Enolase antibody (ab49343)参考文献

This product has been referenced in:
  • Bentaib A  et al. Metabolic reprogramming in transformed mouse cortical astrocytes: A proteomic study. J Proteomics 113C:292-314 (2014). Read more (PubMed: 25305589) »
  • Deng MY  et al. Frontal-Subcortical Protein Expression following Prenatal Exposure to Maternal Inflammation. PLoS One 6:e16638 (2011). WB ; Mouse . Read more (PubMed: 21347362) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Namalwa)
Loading amount 20 µg
Specification Namalwa
Gel Running Conditions Reduced Denaturing (2D gel)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

提交于 May 07 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (embryonic brain slice)
Specification embryonic brain slice
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X in 0.1M PBS
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Nov 14 2007