概述

  • 产品名称
  • 描述
    兔多克隆抗体to NOLC1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 641-690 (DNSFDAKRGA AGDWGERANQ VLKFTKGKSF RHEKTKKKRG SYRGGSISVQ) of Human NOLC1 (NP_004732).

  • 阳性对照
    • HepG2 cell lysate. Human kidney.

性能

应用

Our Abpromise guarantee covers the use of ab106324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 74 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.
  • 序列相似性
    Contains 1 LisH domain.
  • 翻译后修饰
    Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. There is evidence suggesting that CDK1 kinase phosphorylates p130 at the M-phase.
  • 细胞定位
    Nucleus > nucleolus. Cytoplasm. Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 140 kDa nucleolar phosphoprotein antibody
    • HCV NS5A trans regulated protein 13 antibody
    • HCV NS5A transactivated protein 13 antibody
    • HCV NS5A-transactivated protein 13 antibody
    • Hepatitis C virus NS5A transactivated protein 13 antibody
    • Hepatitis C virus NS5A-transactivated protein 13 antibody
    • KIAA0035 antibody
    • NOLC 1 antibody
    • NOLC1 antibody
    • NOLC1_HUMAN antibody
    • NOPP 130 antibody
    • NOPP 140 antibody
    • NOPP130 antibody
    • Nopp140 antibody
    • NS5ATP13 antibody
    • Nucleolar 130 kDa protein antibody
    • Nucleolar and coiled body phosphoprotein 1 antibody
    • Nucleolar and coiled body phosphprotein 1 antibody
    • Nucleolar and coiled-body phosphoprotein 1 antibody
    • Nucleolar phosphoprotein p130 antibody
    • Nucleolar protein p130 antibody
    • P130 antibody
    see all

图片

  • All lanes : Anti-NOLC1 antibody (ab106324) at 2.5 µg/ml

    Lane 1 : Markers
    Lane 2 : HepG2 cell lysate at 10 µg


    Predicted band size : 74 kDa
    12% gel
  • ab106324 at 4-8 µg/ml staining NOLC1 in epithelial cells of the renal tubules (arrowed) in paraffin-embedded Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Magnification is 400X.
  • ab106324 used at a 1/250 dilution staining NOLC1 in zebrafish kidney by Immunohistochemistry (PFA fixed).

文献

ab106324 has not yet been referenced specifically in any publications.

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