Chimpanzee, Rhesus monkey
Synthetic peptide, corresponding to a region within amino acids 163-213 (SLTASLHAHS RKKVKRKHPR RAQDSKKPPR APRTSKAQRR RLTGKARHSG E) of Human NOL12 (NP_077289.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May bind to 28S rRNA.
Belongs to the RRP17 family.
Nucleus > nucleolus.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL12 antibody (ab99276)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NOL12 with ab99276 at 1/1000 (1µg/mg). Detection: DAB.
Western blot - Anti-NOL12 antibody (ab99276)
All lanes : Anti-NOL12 antibody (ab99276) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 25 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-NOL12 antibody (ab99276)
Detection of NOL12 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99276 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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