概述

  • 产品名称Anti-NOL12抗体
    参阅全部 NOL12 一抗
  • 描述
    兔多克隆抗体to NOL12
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 163-213 (SLTASLHAHS RKKVKRKHPR RAQDSKKPPR APRTSKAQRR RLTGKARHSG E) of Human NOL12 (NP_077289.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG

相关产品

应用

Our Abpromise guarantee covers the use of ab99276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-NOL12 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NOL12 with ab99276 at 1/1000 (1µg/mg). Detection: DAB.
  • All lanes : Anti-NOL12 antibody (ab99276) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 25 kDa


    Exposure time : 3 minutes
  • Detection of NOL12 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99276 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-NOL12 antibody (ab99276)参考文献

ab99276 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99276.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"