Anti-Nogo Receptor抗体(ab32890)

概述

  • 产品名称Anti-Nogo Receptor抗体
    参阅全部 Nogo Receptor 一抗
  • 描述
    山羊多克隆抗体to Nogo Receptor
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    LQALP DDTFRDLGN

    , corresponding to Internal sequence amino acids 166-179 of Human Nogo Receptor

  • 阳性对照
    • Human brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab32890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Antibody detection limit dilution 1/16,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa. Gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate and were successfully blocked by the immunizing peptide corresponding to earlier findings in literature (Wang et al).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Nogo Receptor antibody 图像

    • All lanes : Anti-Nogo Receptor antibody (ab32890) at 0.3 µg/ml

      Lane 1 : [A] Human brain lysate in RIPA buffer, without immunising peptide
      Lane 2 : [B] Human brain lysate in RIPA buffer, with immunising peptide

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa,23 kDa,37 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Anti-Nogo Receptor antibody (ab32890)参考文献

    ab32890 has not yet been referenced specifically in any publications.

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    Thank you for contacting us.

    I can confirm that both products are tested and guaranteed for WB in Human samples. That means, if the customer would experience any difficulties we are pleased to help and offer some refund in case the product doe...

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    Thank you for contacting us. The concentration of this antibody is 0.5 mg/ml. This information can be found about halfway down the datasheet.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advic...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"