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Our Abpromise guarantee covers the use of ab26291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 17984503|
|IHC-P||1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
(Image courtesy of Human Protein Atlas)
ab26291 staining Nogo Receptor protein in neuronal cells of normal Human cerebral cortex. Paraffin embedded human cerebral cortex tissue was incubated with ab26291(1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.