Anti-Nogo Receptor抗体(ab26291)

概述

性能

应用

Our Abpromise guarantee covers the use of ab26291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 17984503
IHC-P 1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

Anti-Nogo Receptor antibody 图像

  • Anti-Nogo Receptor antibody (ab26291) at 1 µg/ml + Rat Brain Tissue Lysate (Whole Cell) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 49 kDa (possible truncated form).
  • (Image courtesy of Human Protein Atlas)
    ab26291 staining Nogo Receptor protein in neuronal cells of normal Human cerebral cortex. Paraffin embedded human cerebral cortex tissue was incubated with ab26291(1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

Anti-Nogo Receptor antibody (ab26291)参考文献

This product has been referenced in:
  • Travaglia A  et al. Developmental changes in plasticity, synaptic, glia and connectivity protein levels in rat dorsal hippocampus. Neurobiol Learn Mem 135:125-138 (2016). Read more (PubMed: 27523749) »
  • Theotokis P  et al. Nogo receptor complex expression dynamics in the inflammatory foci of central nervous system experimental autoimmune demyelination. J Neuroinflammation 13:265 (2016). Read more (PubMed: 27724971) »

See all 6 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Motor cortex)
Specification
Motor cortex
Permeabilization
No
Fixative
Paraformaldehyde
Username

Mr. David Pruitt

Verified customer

提交于 Jan 27 2014

Vielen Dank für Ihre Anfrage.

Da wir auf unserer Webseite keine expliziten Angaben unter der WB Applikation gemacht haben, können Sie Ihr Standardt-Protokoll verwenden, da wir nur Protokollempfehlungen bei bekannten Problemen (z.B....

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Wie am Freitag besprochen, möchte ich Ihnen den ab26291 Anti-Nogo Receptor antibody aus unserem Sortiment empfehlen, da nicht nur Ihrer Test- Spezies "...

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Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain Cortex and Hippocampus)
Specification
Brain Cortex and Hippocampus
Username

Dr. Sophie Pezet

Verified customer

提交于 Jul 29 2011




Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab26291 in WB zu benutzen und dann eine Abbildung einzusenden, annehmen möchten. Wie am Telefon besprochen können Sie den Gutscheincode zum Kauf eines ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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