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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Noggin.
(Peptide available as ab16380.)
Our Abpromise guarantee covers the use of ab16054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/175. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26, 35 kDa (predicted molecular weight: 26 kDa).Can be blocked with Human Noggin peptide (ab16380).|
Immunohistochemical analysis of human small intestine tissue, labeling Noggin with ab16054. Tissue was formaldehyde fixed, treated with EDTA (pH 8.6) at 100°C for 20 minutes for heat-mediated antigen retrieval and blocked with 3% Hydrogen Peroxide for 10 minutes at 25°C. Incubation with ab16054 (diluted 1/400) was performed for 20 minutes at 25°C.
Immunohistochemical analysis of mouse kidney tissue, labeling Noggin with ab16054. Tissue was paraformaldehyde fixed, treated with Citrate buffer for heat-mediated antigen retrieval and blocked with Serum Free Protein Block for 20 minutes. Incubation with ab16054 (diluted 1/2500) was performed for 15 hours at 4°C
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