Rat, Chicken, Human
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Noelin.
(Peptide available as ab91078.)
- This antibody gave a positive signal in Mouse Liver tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Seems to play an important role in regulating the production of neural crest cells by the neural tube.
Contains 1 olfactomedin-like domain.
Endoplasmic reticulum lumen.
Information by UniProt
- AMY antibody
- NEUROBLASTOMA PROTEIN antibody
- Neuronal olfactomedin-related ER localized protein antibody
Western blot - Noelin antibody (ab35120)
Anti-Noelin antibody (ab35120) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µgSecondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
55 kDaObserved band size:
60 kDa (why is the actual band size different from the predicted?
)Additional bands at:
43 kDa. We are unsure as to the identity of these extra bands.Exposure time:
Noelin precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
has not yet been referenced specifically in any publications.
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