概述

  • 产品名称Anti-Nodal抗体[EPR2057]
    参阅全部 Nodal 一抗
  • 描述
    兔单克隆抗体[EPR2057] to Nodal
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Nodal.

  • 阳性对照
    • WB: SH-SY5Y and PC-12 cell lysates Flow Cyto: Permeabilized SH-SY5Y cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Essential for mesoderm formation and axial patterning during embryonic development.
    • 疾病相关Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
    • 序列相似性Belongs to the TGF-beta family.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HTX5 antibody
      • MGC138230 antibody
      • Nodal antibody
      • Nodal Growth Differentiation Factor antibody
      • nodal homolog (mouse) antibody
      • Nodal homolog antibody
      • Nodal, Mouse, Homolog antibody
      • NODAL_HUMAN antibody
      see all

    Anti-Nodal antibody [EPR2057] 图像

    • All lanes : Anti-Nodal antibody [EPR2057] (ab109317) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : PC12 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • Flow cytometric analysis of Nodal in permeabilized SH-SY5Y cells, using ab109317 (red) or a rabbit IgG (negative) (green).

    Anti-Nodal antibody [EPR2057] (ab109317)参考文献

    This product has been referenced in:
    • Chen J  et al. Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma. PLoS One 9:e85840 (2014). WB ; Human . Read more (PubMed: 24465741) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting Abcam.

    The immunogen information you requested is listed below for each antibody:
    1. ab39242: immunogen is upstream of residue XXX.
    2. ab39953: immunogen is downstream of residue XXX.
    3. ab109317: immunogen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"