重组
RabMAb

Anti-Nodal抗体[EP2058Y] (ab81287)

概述

  • 产品名称
    Anti-Nodal抗体[EP2058Y]
    参阅全部 Nodal 一抗
  • 描述
    兔单克隆抗体[EP2058Y] to Nodal
  • 特异性
    ab81287 detects Pro-Nodal and Nodal.
  • 经测试应用
    适用于: Flow Cyt, WBmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human Nodal.

  • 阳性对照
    • Recombinant nodal protein.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab81287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/2000. Predicted molecular weight: 39 kDa. (Pro-Nodal) and 13 kDa (Nodal).
  • 应用说明
    Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能
      Essential for mesoderm formation and axial patterning during embryonic development.
    • 疾病相关
      Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
    • 序列相似性
      Belongs to the TGF-beta family.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HTX5 antibody
      • MGC138230 antibody
      • Nodal antibody
      • Nodal Growth Differentiation Factor antibody
      • nodal homolog (mouse) antibody
      • Nodal homolog antibody
      • Nodal, Mouse, Homolog antibody
      • NODAL_HUMAN antibody
      see all

    图片

    • Anti-Nodal antibody [EP2058Y] (ab81287) at 1/2000 dilution + Recombinant nodal protein at 10ng

      Secondary
      HRP conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 39 kDa
      Observed band size : 15 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Strizzi L  et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget 6:34071-86 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26460952) »
    • Malchenko S  et al. Cancer hallmarks in induced pluripotent cells: new insights. J Cell Physiol 225:390-3 (2010). WB ; Human . Read more (PubMed: 20568225) »

    See all 2 Publications for this product

    客户评价及客户问答

    Thank you for contacting Abcam.

    The immunogen for ab81287 is downstream of Nodal residue XXX.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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