重组Anti-Nodal抗体[EP2058Y] (ab81287)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2058Y] to Nodal
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Nodal抗体[EP2058Y]
参阅全部 Nodal 一抗 -
描述
兔单克隆抗体[EP2058Y] to Nodal -
宿主
Rabbit -
特异性
ab81287 detects Pro-Nodal and Nodal. This antibody is unable to detect signal in endogenous samples.
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经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human Nodal aa 300-400 (C terminal). The exact sequence is proprietary.
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阳性对照
- Recombinant nodal protein.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2058Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81287于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000. Predicted molecular weight: 39 kDa.
(Pro-Nodal) and 13 kDa (Nodal). |
说明 |
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WB
1/2000. Predicted molecular weight: 39 kDa. (Pro-Nodal) and 13 kDa (Nodal). |
靶标
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功能
Essential for mesoderm formation and axial patterning during embryonic development. -
疾病相关
Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4838 Human
- Entrez Gene: 18119 Mouse
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- SwissProt: P43021 Mouse
- Unigene: 370414 Human
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别名
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
图片
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Anti-Nodal antibody [EP2058Y] (ab81287) at 1/2000 dilution + Recombinant nodal protein at 10ng
Secondary
HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab81287 被引用在 3 文献中.
- Li H et al. Nodal induced by hypoxia exposure contributes to dacarbazine resistance and the maintenance of stemness in melanoma cancer stem-like cells. Oncol Rep 39:2855-2864 (2018). PubMed: 29693169
- Strizzi L et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget 6:34071-86 (2015). Sandwich ELISA ; Human . PubMed: 26460952
- Malchenko S et al. Cancer hallmarks in induced pluripotent cells: new insights. J Cell Physiol 225:390-3 (2010). WB ; Human . PubMed: 20568225