概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to Nodal
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: RCEGECPNPV GEEFHPTNHA YIQSLLKRYQ PHRVPSTCCA PVKTKPLSML YVDNGRVLLD HHKDMIVEEC GC, corresponding to amino acids 275-347 of Human Nodal

性能

应用

Our Abpromise guarantee covers the use of ab55676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Essential for mesoderm formation and axial patterning during embryonic development.
  • 疾病相关
    Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
  • 序列相似性
    Belongs to the TGF-beta family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HTX5 antibody
    • MGC138230 antibody
    • Nodal antibody
    • Nodal Growth Differentiation Factor antibody
    • nodal homolog (mouse) antibody
    • Nodal homolog antibody
    • Nodal, Mouse, Homolog antibody
    • NODAL_HUMAN antibody
    see all

Anti-Nodal antibody 图像

  • Nodal antibody (ab55676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.


  • Predicted band size : 40 kDa
    Nodal antibody (ab55676) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab55676 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55676, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HEK293 cells stained with ab55676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55676, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Nodal antibody (ab55676)参考文献

This product has been referenced in:
  • Strizzi L  et al. Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi. Oncol Lett 12:1349-1354 (2016). IHC . Read more (PubMed: 27446436) »
  • Chen J  et al. Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma. PLoS One 9:e85840 (2014). IHC-P ; Human . Read more (PubMed: 24465741) »

See all 5 Publications for this product

Product Wall

Thank you for your reply.

I am sorry about the confusion whether ab55676 was tested in IF or not, as you say the image on the datasheet clearly shows that is does work in IF, I am sorry I missed that.

Having, looked over your protoc...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (1 week Retinoic acid differentiation of NTERA-2)
Specification
1 week Retinoic acid differentiation of NTERA-2
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 21 2015

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Human Cell lysate - whole cell (LNCaP cell line)
Specification
LNCaP cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

提交于 May 15 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
CAS BLOCK as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Sample
Human Tissue sections (Human Prostate)
Specification
Human Prostate
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Francesco Elia Marino

Verified customer

提交于 May 15 2014

Thank you for your reply.

I am sorry that I misread your original email with the protocol information, I know see that you tried the secondary antibody with both 15 and 30 minutes incubation times but that did not make a difference.

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Thank you for taking the time to respond and for contacting Abcam.

With over 70,000 products it is logistically impossible for us to offer free samples. I hope you understand.

Please let me know if you have any questions.

Thank you for contacting Abcam.

I am sorry that you are having problems with ab55676 in immunofluorescence. As we had not tested this antibody in IF before, we were not sure how the antibody would work in that application. I would be very hap...

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Thank you for contacting Abcam.

I am sorry to hear that Ab55676 has not performed well for you. Although the peptide sequence has excellent identity with the mouse homolog, this product is not guaranteed to work in mouse tissues. High backgro...

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Further to my previous response to your complaint, I would like to pass some more information which you may find useful. The antibody is (NODAL monoclonal antibody (ab55676) has been tested and characterized in Western blot on several cell lines, such ...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. NODAL is essential for mesoderm formation and axial pat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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