Anti-Nodal抗体(ab39953)

概述

  • 产品名称Anti-Nodal抗体
    参阅全部 Nodal 一抗
  • 描述
    兔多克隆抗体to Nodal
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Nodal.

    (Peptide available as ab39952.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; MEF1; F9; Mouse EG; SHSY-5Y.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Essential for mesoderm formation and axial patterning during embryonic development.
    • 疾病相关Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
    • 序列相似性Belongs to the TGF-beta family.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HTX5 antibody
      • MGC138230 antibody
      • Nodal antibody
      • Nodal Growth Differentiation Factor antibody
      • nodal homolog (mouse) antibody
      • Nodal homolog antibody
      • Nodal, Mouse, Homolog antibody
      • NODAL_HUMAN antibody
      see all

    Anti-Nodal antibody 图像

    • ICC/IF image of ab39953 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39953, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • All lanes : Anti-Nodal antibody (ab39953) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 4 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
      Lane 5 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
      Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to rabbit IgG -H&L - pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
      Additional bands at : 70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Anti-Nodal antibody (ab39953)参考文献

    ab39953 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam.

    The immunogen information you requested is listed below for each antibody:
    1. ab39242: immunogen is upstream of residue XXX.
    2. ab39953: immunogen is downstream of residue XXX.
    3. ab109317: immunogen...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (whole embryo E6.5)
    Specification whole embryo E6.5
    Fixative Paraformaldehyde
    Permeabilization Yes - PBtriton-x-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 110% · Temperature: RT°C
    Username

    Dr. T Rodriguez

    Verified customer

    提交于 Aug 14 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"