• 产品名称Anti-Nocturnin抗体
  • 描述
    兔多克隆抗体to Nocturnin
  • 特异性Although there is a high homology with mouse, this antibody also has the potential to cross react with another mouse protein of similar molecular weight therefore this antibody is not suitable for use in mouse.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow
  • 免疫原

    A region within a synthetic peptide: RLYSALAKTL NSSAASQHPE YLVSPDPEHL EPIDPKELLE ECRAVLHTRP, corresponding to N terminal amino acids 73-122 of Human Nocturnin

  • 阳性对照
    • Human lung tissue (IHC-P), Daudi cell lysate (western blot).


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab22850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • 相关性CCRN4L is highly similar to Nocturnin, a gene identified as a circadian clock regulated gene in Xenopus laevis. This protein and Nocturnin protein share similarity with the C terminal domain of a yeast transcription factor, carbon catabolite repression 4 (CCR4). The mRNA abundance of a similar gene in mouse has been shown to exhibit circadian rhythmicity, which suggests a role for this protein in clock function or as a circadian clock effector.
  • 数据库链接
  • 别名
    • CCR4 carbon catabolite repression 4 like (S. cerevisiae) antibody
    • CCR4 protein homolog antibody
    • CCRN4L antibody
    • NOC antibody

Anti-Nocturnin antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Nocturnin with ab22850 at 4.0-8.0µg/ml. Positive staining seen in the bronchiole epithelium. Magnification: 400X.
  • Anti-Nocturnin antibody (ab22850) at 4 µg/ml + Daudi cell lysate

    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Paraffin-embedded Human lung tissue. Cells with positive label: epithelial cells of bronchiole (indicated by arrows). Antibody concentration: 4-8µg/ml. Magnification: 400x.

Anti-Nocturnin antibody (ab22850)参考文献

ab22850 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cells (fibroblasts))
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification 3T3 cells (fibroblasts)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jun 10 2015

Thank you for getting back to me with further information. I have read your previous questionnaire and the further details that you have provided me with. I find your enquiry most interesting. As I can see you are ONLY detecting the correctly sized...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. You mention that this antibody has been detecting bands other than ove...

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