Nocodazole,Microtubule抑制剂(ab120630)
Key features and details
- Microtubule inhibitor
- CAS Number: 31430-18-9
- Purity: > 98%
Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Nocodazole,Microtubule抑制剂 -
描述
Microtubule抑制剂 -
纯度
> 98% -
CAS编号
31430-18-9 -
化学结构
性能
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化学名称
Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate
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分子量
301.32 -
分子式
C14H11N3O3S -
PubChem识别号
4122 -
存放说明
Store at +4°C. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 50 mM
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处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3 -
来源
Synthetic
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研究领域
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
图片
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2D chemical structure image of ab120630, Nocodazole, Microtubule inhibitor
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All lanes : Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 23 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate 10 μg (Input).
Lane 2: ab217673 IP in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab120630 被引用在 13 文献中.
- Endo Y et al. Genome‑wide DNA hypomethylation drives a more invasive pancreatic cancer phenotype and has predictive occult distant metastasis and prognosis potential. Int J Oncol 60:N/A (2022). PubMed: 35419613
- Yan H et al. CDK5RAP3, an essential regulator of checkpoint, interacts with RPL26 and maintains the stability of cell growth. Cell Prolif 55:e13240 (2022). PubMed: 35509151
- Lee BY & Hur EM A Role of Microtubules in Oligodendrocyte Differentiation. Int J Mol Sci 21:N/A (2020). PubMed: 32033476
- Wu B et al. Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells. Cell Metab 32:967-980.e5 (2020). PubMed: 33264602
- Sugita S et al. Tumor-suppressive microRNA-223 targets WDR62 directly in bladder cancer. Int J Oncol 54:2222-2236 (2019). PubMed: 30942440