Anti-NNT抗体[8B4BB10] (ab110352)
Key features and details
- Mouse monoclonal [8B4BB10] to NNT
- Suitable for: IP, ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-NNT抗体[8B4BB10]
参阅全部 NNT 一抗 -
描述
小鼠单克隆抗体[8B4BB10] to NNT -
宿主
Mouse -
特异性
Several customers have found that this antibody may not work well for mouse and rat samples.
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经测试应用
适用于: IP, ICC/IF, IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Normal Human skin fibroblasts (strain HDFn); Human cerebellum tissue; mitochondria from Human liver, Bovine heart, Rat liver and Mouse liver; HepG2 and HL-60 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
8B4BB10 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110352于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P |
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 114 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 114 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. -
序列相似性
In the N-terminal section; belongs to the AlaDH/PNT family.
In the C-terminal section; belongs to the PNT beta subunit family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 280878 Cow
- Entrez Gene: 23530 Human
- Omim: 607878 Human
- SwissProt: P11024 Cow
- SwissProt: Q13423 Human
- SwissProt: Q61941 Mouse
- Unigene: 482043 Human
- Unigene: 195803 Mouse
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别名
- GCCD4 antibody
- mitochondrial antibody
- NAD(P) transhydrogenase antibody
see all
图片
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Anti-NNT antibody [8B4BB10] (ab110352) at 0.5 µg/ml + HepG2 whole cell lysate
Predicted band size: 114 kDa
anti-Complex II 30kDa subunit has also been labeled as a control. -
HL-60 cells were stained with 1 µg/mL NNT antibody (ab110352) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Mitochondrial localization of NNT in cultured, normal Human skin fibroblasts (strain HDFn) labeled with anti NNT (ab110352) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody.
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NNT immunohistochemistry in Human cerebellum visualized with ab110352 at 1/100. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
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Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from Human liver mitochondria (lane 1), bovine heart mitochondria (lane 2), rat liver mitochondria (lane 3), mouse liver mitochondria (lane 4), and HepG2 cells (lane 5), using ab110352.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab110352 被引用在 6 文献中.
- Bharath LP et al. Metformin Enhances Autophagy and Normalizes Mitochondrial Function to Alleviate Aging-Associated Inflammation. Cell Metab 32:44-55.e6 (2020). PubMed: 32402267
- Ward NP et al. Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function. J Exp Med 217:N/A (2020). PubMed: 32196080
- McCambridge G et al. Saturated Fatty Acid Activates T Cell Inflammation Through a Nicotinamide Nucleotide Transhydrogenase (NNT)-Dependent Mechanism. Biomolecules 9:N/A (2019). PubMed: 30823587
- Ren W et al. Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method. Molecules 23:N/A (2018). PubMed: 30041436
- Dey S et al. Compartment-specific Control of Reactive Oxygen Species Scavenging by Antioxidant Pathway Enzymes. J Biol Chem 291:11185-97 (2016). PubMed: 27048652
- Agarwal AR et al. Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs. Am J Physiol Lung Cell Mol Physiol 303:L889-98 (2012). WB ; Mouse . PubMed: 23064950