Anti-nNOS (neuronal)抗体(ab79051)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-nNOS (neuronal)抗体
参阅全部 nNOS (neuronal) 一抗 -
描述兔多克隆抗体to nNOS (neuronal)
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宿主Rabbit
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经测试应用适用于: WB, IP, IHC-P, ICCmore details
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种属反应性与反应: Rat, Human
预测可用于: Mouse, Rabbit
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免疫原
Synthetic peptide:
ESKKDTDEVFSS
, corresponding to amino acids 1422-1433 of Human nNOS (neuronal) -
阳性对照
- Skeletal muscle tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab79051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 161 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC | Use at an assay dependent concentration. |
靶标
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功能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
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组织特异性Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
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序列相似性Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
结构域The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
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翻译后修饰Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
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细胞定位Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
- Information by UniProt
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数据库链接
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Entrez Gene: 24598 Rat
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
- SwissProt: O19132 Rabbit
see all -
别名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - nNOS (neuronal) antibody (ab79051)Immunohistochemical analysis of nNOS (neuronal) in formalin-fixed, paraffin-embedded human skeletal tissue using ab79051 at 5µg/ml.
实验方案
文献
This product has been referenced in:
- Lopez M et al. Tumor necrosis factor and transforming growth factor ß regulate clock genes by controlling the expression of the cold inducible RNA-binding protein (CIRBP). J Biol Chem 289:2736-44 (2014). Read more (PubMed: 24337574) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"