概述

  • 产品名称Anti-nNOS (neuronal)抗体
    参阅全部 nNOS (neuronal) 一抗
  • 描述
    兔多克隆抗体to nNOS (neuronal)
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from Human nNOS (neuronal) around the phosphorylation site of serine 852 (F-N-SP-V-S).

  • 阳性对照
    • HeLa cells and extracts from RAW264.7 cells treated with INF (2500u/ml, 10mins).

性能

应用

Our Abpromise guarantee covers the use of ab63602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately >117, 95 kDa (predicted molecular weight: 161 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 组织特异性Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • 序列相似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • 翻译后修饰Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • 细胞定位Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Anti-nNOS (neuronal) antibody 图像

  • ab63602, at a 1/500 dilution, staining Human nNos (neuronal) in HeLa cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
  • All lanes : Anti-nNOS (neuronal) antibody (ab63602) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract treated with INF (2500u/ml, 10mins)
    Lane 2 : RAW264.7 cell extract treated with INF (2500u/ml, 10mins) with the immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 161 kDa
    Observed band size : >117 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

Anti-nNOS (neuronal) antibody (ab63602)参考文献

This product has been referenced in:
  • Safdar A  et al. Aberrant mitochondrial homeostasis in the skeletal muscle of sedentary older adults. PLoS One 5:e10778 (2010). WB ; Human . Read more (PubMed: 20520725) »

See 1 Publication for this product

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