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Our Abpromise guarantee covers the use of ab3511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 22872659|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/400.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skeletal muscle tissue sections labeling nNOS (neuronal) with ab3511 at 1/400 (left). Antigen retrieval was performed using 10M sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA in PBS for 30 minutes at room temperature. A biotin-conjugated secondary antibody was used. Right - negative control.
ab3511 at 1/800 dilution staining nNOS (neuronal) in rat brain tissue section by immunohistochemistry (Frozen sections).