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Our Abpromise guarantee covers the use of ab15203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20207227|
|IHC-P||1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 150 kDa.|
ab15203 staining nNOS (neuronal) in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Sections were deparaffinized, rehydrated and submitted to antigen retrieval and blocked with 5% non-fat dry milk and 0.1% Triton X-100. Samples were incubated with primary antibody (1/200 in PBS containing 2% non-fat dry milk and 0.05% Trition X-00) overnight at 4°C. A Biotin-conjugated anti-rabbit IgG (1/200 in PBS containing 1% NGS for 1 hour at room temperature) was used as the secondary antibody. Left - wild type, Right - Cre+/RTN-1C+. Magnification: 20x.
Human placenta tissue section stained with ab15203 by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
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