为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Synthetic peptide corresponding to Mouse nNOS (neuronal) (C terminal).
Our Abpromise guarantee covers the use of ab15203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20207227|
|IHC-P||1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 150 kDa.|
ab15203 staining nNOS (neuronal) in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Sections were deparaffinized, rehydrated and submitted to antigen retrieval and blocked with 5% non-fat dry milk and 0.1% Triton X-100. Samples were incubated with primary antibody (1/200 in PBS containing 2% non-fat dry milk and 0.05% Trition X-00) overnight at 4°C. A Biotin-conjugated anti-rabbit IgG (1/200 in PBS containing 1% NGS for 1 hour at room temperature) was used as the secondary antibody. Left - wild type, Right - Cre+/RTN-1C+. Magnification: 20x.
Human placenta tissue section stained with ab15203 by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).