Anti-nNOS (neuronal)抗体(ab1376)

概述

  • 产品名称Anti-nNOS (neuronal)抗体
    参阅全部 nNOS (neuronal) 一抗
  • 描述
    山羊多克隆抗体to nNOS (neuronal)
  • 经测试应用适用于: IHC-FrFl, WB, IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human, Marmoset (common)
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.

  • 阳性对照
    • WB: mouse brain lysate IHC: mouse DRG following spinal nerve ligation (2 weeks)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab1376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FrFl Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45, 160 kDa (predicted molecular weight: 161 kDa).Can be blocked with Human nNOS (neuronal) peptide (ab22863).

Lyse in RIPA buffer.

IHC-FoFr 1/300. Ovenight incubation at room temp.
IHC-P Use at an assay dependent concentration. PubMed: 16263087
IHC-Fr 1/1000. PubMed: 22589245

靶标

  • 功能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 组织特异性Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • 序列相似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • 翻译后修饰Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • 细胞定位Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Anti-nNOS (neuronal) antibody 图像

  • Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded marmoset cerebellum tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

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  • ab1376 nNos antibody immunostaining nNos in dorsal root ganglion (DRG) neurons of an animal who had a spinal nerve ligation 2 weeks previous. [A] Image taken X10 objective, [B]Image taken with X20 objective; the staining is restricted to a subpopulation of neurons and is localized in the cytoplasm of these cells; Scale bar=20 microns. ab1376 was used at 1/300 overnight incubation at room temperature on 30 micron sections of PFA perfusion fixed DRG tissue (on slide). Staining visualised using anti goat FITC secondary.
  • Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded rat cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

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  • IHC-Fr image of nNOS staining on marmoset caudate sections using ab1376 (1:1000). The tissue was fixed in paraformaldehyde and the sections were then permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab1376 was diluted 1:1000 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Rabbit polyclonal to Goat IgG (undiluted)

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  • Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded mouse cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

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  • Predicted band size : 161 kDa
    ab1376 (0.3 µg/ml) staining nNOS (neuronal) in human muscle whole tissue lysate (35 µg in RIPA). Incubation for 1 hour; detected by chemiluminescence.

Anti-nNOS (neuronal) antibody (ab1376)参考文献

This product has been referenced in:
  • Khan M  et al. Blocking a vicious cycle nNOS/peroxynitrite/AMPK by S-nitrosoglutathione: implication for stroke therapy. BMC Neurosci 16:42 (2015). WB ; Rat . Read more (PubMed: 26174015) »
  • Li G  et al. Firing of hippocampal neurogliaform cells induces suppression of synaptic inhibition. J Neurosci 34:1280-92 (2014). Read more (PubMed: 24453319) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (stomach)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate PH 6,6
Permeabilization Yes - Triton X100 0.5%
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mr. Julien Chevalier

Verified customer

提交于 Aug 08 2016

Application IHC - Wholemount
Sample Pig Tissue (Intestinal myenteric plexus)
Specification Intestinal myenteric plexus
Username

Mr. Julien Chevalier

Verified customer

提交于 Aug 08 2016

Application IHC - Wholemount
Sample Human Tissue (Intestinal myenteric plexus)
Specification Intestinal myenteric plexus
Username

Abcam user community

Verified customer

提交于 Aug 08 2016

Application IHC - Wholemount
Sample Rat Tissue (Intestinal myenteric plexus)
Specification Intestinal myenteric plexus
Username

Mr. Julien Chevalier

Verified customer

提交于 Aug 08 2016

Application IHC - Wholemount
Sample Mouse Tissue (Intestinal myenteric plexus)
Specification Intestinal myenteric plexus
Username

Mr. Julien Chevalier

Verified customer

提交于 Aug 08 2016

Application Western blot
Sample Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 18 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Jugular-Nodose ganglia)
Permeabilization No
Specification Jugular-Nodose ganglia
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

提交于 Jun 08 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 21 2015

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