概述

  • 产品名称Anti-nNav1.5抗体[4G8:1G7]
  • 描述
    小鼠单克隆抗体[4G8:1G7] to nNav1.5
  • 特异性The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant.
  • 经测试应用适用于: ICC/IF, WB, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human nNav1.5.

性能

应用

Our Abpromise guarantee covers the use of ab62388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 227 kDa.
ICC Use a concentration of 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Anti-nNav1.5 antibody [4G8:1G7] 图像

  • Ab62388 (10ug/ml) staining human Nav1.5 in Nav1.5-HEK293 recombinant cell line by immunocytochemistry.

    A: Plasma membrane localisation of Nav1.5 identified by ab62388 detected by anti-mouse Alexa Fluor-488 conjugated antibody.

    B: DAP1 co-staining of cell nuclei.
  • Overlay histogram showing SHSY-5Y cells stained with ab62388 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62388, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879>) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-nNav1.5 antibody [4G8:1G7] (ab62388)参考文献

This product has been referenced in:
  • Guo L  et al. Estimating the risk of drug-induced proarrhythmia using human induced pluripotent stem cell-derived cardiomyocytes. Toxicol Sci 123:281-9 (2011). ICC/IF ; Human . Read more (PubMed: 21693436) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry
Sample Dog Cultured Cells (mammary, tumoral)
Permeabilization No
Specification mammary, tumoral
Fixative Methanol
Username

Dr. Martina Argano

Verified customer

提交于 Aug 07 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hESC-derived cardiomyocyte)
Specification hESC-derived cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"