Anti-nmt55 / p54nrb抗体(ab50952)

概述

  • 产品名称Anti-nmt55 / p54nrb抗体
    参阅全部 nmt55 / p54nrb 一抗
  • 描述
    兔多克隆抗体to nmt55 / p54nrb
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    A region within synthetic peptide: GTLGLTPPTT ERFGQAATME GIGAIGGTPP AFNRAAPGAE FAPNKRRRY, corresponding to C terminal amino acids 423-471 of Human nmt55/ p54nrb

  • 阳性对照
    • HepG2 cell lysate; paraffin-embedded human liver tissue, paraffin-embedded human kidney tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
  • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
  • 疾病相关Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 52 kDa subunit antibody
    • 54 kDa nuclear RNA and DNA binding protein antibody
    • 54 kDa nuclear RNA- and DNA-binding protein antibody
    • 55 kDa nuclear protein antibody
    • DNA binding p52/p100 complex 52 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • NMT 55 antibody
    • NMT55 antibody
    • Non Pou domain containing octamer (ATGCAAAT) binding protein antibody
    • Non POU domain containing octamer binding antibody
    • Non POU domain containing octamer binding protein antibody
    • Non-POU domain-containing octamer-binding protein antibody
    • Nono antibody
    • NonO protein antibody
    • NONO_HUMAN antibody
    • NRB 54 antibody
    • NRB antibody
    • NRB54 antibody
    • Nuclear RNA binding protein 54kD antibody
    • P54 antibody
    • p54(nrb) antibody
    • p54nrb antibody
    • PPP1R114 antibody
    • Protein phosphatase 1 regulatory subunit 114 antibody
    see all

Anti-nmt55 / p54nrb antibody 图像

  • Anti-nmt55 / p54nrb antibody (ab50952) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Tissue: paraffin-embedded human Liver
    Cells with positive label: Hepatocytes (indicated with arrows)
    Antibody concentration: 4 - 8 µg/ml
    Magnification: 400X
  • Tissue: paraffin-embedded human Kidney
    Cells with positive label: Epithelial cells of renal tubule (indicated with arrows)
    Antibody concentration: 4 - 8 µg/ml
    Magnification: 400X
  • ab50952 staining nmt55 / p54nrb in Hela cells by Immunocytochemistry/ Immunofluorescence.

Anti-nmt55 / p54nrb antibody (ab50952)参考文献

ab50952 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human brain motor cortex)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Specification Human brain motor cortex
Blocking step (agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Formalin
Username

Guinevere Lourenco

Verified customer

提交于 Nov 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"