概述

  • 产品名称Anti-nmt55 / p54nrb抗体
    参阅全部 nmt55 / p54nrb 一抗
  • 描述
    山羊多克隆抗体to nmt55 / p54nrb
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    C-NRAAPGAEFAPNK

    , corresponding to C terminal amino acids 455-467 of Human nmt55/ p54nrb

  • 阳性对照
    • Mouse Brain lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

靶标

  • 功能DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
  • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
  • 疾病相关Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 52 kDa subunit antibody
    • 54 kDa nuclear RNA and DNA binding protein antibody
    • 54 kDa nuclear RNA- and DNA-binding protein antibody
    • 55 kDa nuclear protein antibody
    • DNA binding p52/p100 complex 52 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • NMT 55 antibody
    • NMT55 antibody
    • Non Pou domain containing octamer (ATGCAAAT) binding protein antibody
    • Non POU domain containing octamer binding antibody
    • Non POU domain containing octamer binding protein antibody
    • Non-POU domain-containing octamer-binding protein antibody
    • Nono antibody
    • NonO protein antibody
    • NONO_HUMAN antibody
    • NRB 54 antibody
    • NRB antibody
    • NRB54 antibody
    • Nuclear RNA binding protein 54kD antibody
    • P54 antibody
    • p54(nrb) antibody
    • p54nrb antibody
    • PPP1R114 antibody
    • Protein phosphatase 1 regulatory subunit 114 antibody
    see all

Anti-nmt55 / p54nrb antibody 图像

  • Anti-nmt55 / p54nrb antibody (ab50411) at 0.1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).

    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-nmt55 / p54nrb antibody (ab50411) at 1/2000 dilution

    Lane 1 : MEF whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Donkey anti-goat IgG HRP

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • ab50411 at 3.8 µg/ml staining nmt55 / p54nrb in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

Anti-nmt55 / p54nrb antibody (ab50411)参考文献

This product has been referenced in:
  • Rajesh C  et al. The splicing-factor related protein SFPQ/PSF interacts with RAD51D and is necessary for homology-directed repair and sister chromatid cohesion. Nucleic Acids Res 39:132-45 (2011). WB ; Mouse . Read more (PubMed: 20813759) »
  • Ong SA  et al. Rasd1 Modulates the Coactivator Function of NonO in the Cyclic AMP Pathway. PLoS One 6:e24401 (2011). WB ; Human . Read more (PubMed: 21915321) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 50 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jan 18 2008

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