Anti-NMNAT2抗体(ab110040)
Key features and details
- Rabbit polyclonal to NMNAT2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-NMNAT2抗体
参阅全部 NMNAT2 一抗 -
描述
兔多克隆抗体to NMNAT2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Rat Cortex; Mouse Cortex; Rat Forebrain. ICC/IF: This antibody gave a positive result in IF in the following Methanol fixed cell line: SKNSH
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110040于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannnot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). -
组织特异性
Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans. -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1. -
序列相似性
Belongs to the eukaryotic NMN adenylyltransferase family. -
细胞定位
Cytoplasm. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 23057 Human
- Entrez Gene: 226518 Mouse
- Entrez Gene: 289095 Rat
- Omim: 608701 Human
- SwissProt: Q9BZQ4 Human
- SwissProt: Q8BNJ3 Mouse
- SwissProt: Q0HA29 Rat
- Unigene: 497123 Human
see all -
别名
- C1orf15 antibody
- KIAA0479 antibody
- MGC2756 antibody
see all
图片
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All lanes : Anti-NMNAT2 antibody (ab110040) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Mouse Cortex Tissue Lysate
Lane 5 : Rat Forebrain Rat Tissue Lysate
Lane 6 : Lung (Rat) Tissue Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Both Rat Lung tissue lysate and NIH3T3 whole cell lysate were used as negative controls for Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) -
ab110040 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110040 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab110040 被引用在 1 文献中.
- Ali YO et al. Screening with an NMNAT2-MSD platform identifies small molecules that modulate NMNAT2 levels in cortical neurons. Sci Rep 7:43846 (2017). PubMed: 28266613