概述

  • 产品名称
  • 描述
    兔多克隆抗体to Nmnat1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Nmnat1. Note: the amino acid sequence is proprietary)(Peptide available as ab45547.)

  • 阳性对照
    • ab45548 gave a positive result in the following human tissue lysates: Brain, Testis, Liver, Heart, Skeletal Muscle, Kidney, Colon (data not shown).

性能

应用

Our Abpromise guarantee covers the use of ab45548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 29 kDa (predicted molecular weight: 32 kDa).

靶标

  • 功能
    Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults.
  • 组织特异性
    Widely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain.
  • 通路
    Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
    Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.
  • 疾病相关
    Leber congenital amaurosis 9
  • 序列相似性
    Belongs to the eukaryotic NMN adenylyltransferase family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.7.1 antibody
    • LCA9 antibody
    • Leber's congenital amaurosis 9 antibody
    • NaMN adenylyltransferase 1 antibody
    • nicotinamide nucleotide adenylyltransferase 1 antibody
    • Nicotinamide-nucleotide adenylyltransferase 1 antibody
    • Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 antibody
    • nicotinate nucleotide adenylyltransferase 1 antibody
    • Nicotinate-nucleotide adenylyltransferase 1 antibody
    • NMN adenylyltransferase 1 antibody
    • NMN/NaMN adenylyltransferase 1 antibody
    • NMNA1_HUMAN antibody
    • Nmnat 1 antibody
    • Nmnat1 antibody
    • OTTHUMP00000001731 antibody
    • OTTHUMP00000001732 antibody
    • OTTHUMP00000035892 antibody
    • PNAT 1 antibody
    • PNAT1 antibody
    • pyridine nucleotide adenylyltransferase 1 antibody
    see all

图片

  • All lanes : Anti-Nmnat1 antibody (ab45548) at 1/250 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 6 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Giammona LM  et al. Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibition. Exp Hematol 37:1340-1352.e3 (2009). WB ; Human . Read more (PubMed: 19715739) »

See 1 Publication for this product

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