兔单克隆抗体[EP1858Y] to NMDAR2B (phospho S1303)
ab81271 detects NMDAR2B when phosphorylated at serine 1303
Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
Is unsuitable for IHC-P or IP.
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Information by UniProt
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Western blot - NMDAR2B (phospho S1303) antibody [EPR1858Y] (ab81271)
All lanes :
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 :
mouse brain lysates (untreated)
Lane 2 :
mouse brain lysates (treated with AP)
Lysates/proteins at 10 µg per lane.
Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size :
Observed band size :
180 kDa (
why is the actual band size different from the predicted?
This product has been referenced in:
et al. Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain Behav 7:e00734 (2017).
Read more (PubMed: 28729940) »
et al. Differential roles of hippocampal glutamatergic receptors in neuropathic anxiety-like behavior after partial sciatic nerve ligation in rats. BMC Neurosci 16:14 (2015).
Read more (PubMed: 25884414) »
See all 5 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"