重组
RabMAb

Anti-NMDAR2B (phospho S1303)抗体[EP1858Y] (ab81271)

概述

  • 产品名称
    Anti-NMDAR2B (phospho S1303)抗体[EP1858Y]
    参阅全部 NMDAR2B 一抗
  • 描述
    兔单克隆抗体[EP1858Y] to NMDAR2B (phospho S1303)
  • 宿主
    Rabbit
  • 特异性
    ab81271 detects NMDAR2B when phosphorylated at serine 1303
  • 经测试应用
    适用于: WB, ICCmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.

  • 阳性对照
    • WB: Human, mouse and rat brain tissue lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab81271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
ICC 1/100.
  • 应用说明
    Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
    • 组织特异性
      Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
    • 序列相似性
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
    • 细胞定位
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AW490526 antibody
      • EIEE27 antibody
      • Glutamate [NMDA] receptor subunit epsilon 2 antibody
      • Glutamate [NMDA] receptor subunit epsilon-2 antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
      • Glutamate receptor ionotropic NMDA2B antibody
      • Glutamate receptor subunit epsilon 2 antibody
      • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
      • GRIN 2B antibody
      • GRIN2B antibody
      • hNR 3 antibody
      • hNR3 antibody
      • MGC142178 antibody
      • MGC142180 antibody
      • MRD6 antibody
      • N methyl D asparate receptor channel subunit epsilon 2 antibody
      • N methyl D aspartate receptor subtype 2B antibody
      • N methyl D aspartate receptor subunit 2B antibody
      • N methyl D aspartate receptor subunit 3 antibody
      • N-methyl D-aspartate receptor subtype 2B antibody
      • N-methyl-D-aspartate receptor subunit 3 antibody
      • NMDA NR2B antibody
      • NMDA R2B antibody
      • NMDAR2B antibody
      • NMDE2 antibody
      • NMDE2_HUMAN antibody
      • NME2 antibody
      • NR2B antibody
      • NR3 antibody
      see all

    图片

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Mouse brain lysates (untreated)
      Lane 2 : Mouse brain lysates (treated with AP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Castany S  et al. Critical role of sigma-1 receptors in central neuropathic pain-related behaviours after mild spinal cord injury in mice. Sci Rep 8:3873 (2018). Read more (PubMed: 29497125) »
    • Wang Y  et al. Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain Behav 7:e00734 (2017). Read more (PubMed: 28729940) »

    See all 7 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab81271.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册