Anti-NMDAR2B (phospho S1303)抗体[EP1858Y] (ab81271)


  • 产品名称Anti-NMDAR2B (phospho S1303)抗体[EP1858Y]
    参阅全部 NMDAR2B 一抗
  • 描述
    兔单克隆抗体[EP1858Y] to NMDAR2B (phospho S1303)
  • 特异性ab81271 detects NMDAR2B when phosphorylated at serine 1303
  • 经测试应用适用于: WB, ICCmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.

  • 阳性对照
    • Mouse brain lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


    Alternative versions available:

    Anti-NMDAR2B (phospho S1303) antibody (HRP) [EP1858Y] (ab196974)



Our Abpromise guarantee covers the use of ab81271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
ICC 1/100.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
    • 组织特异性Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
    • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
    • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AW490526 antibody
      • EIEE27 antibody
      • Glutamate [NMDA] receptor subunit epsilon 2 antibody
      • Glutamate [NMDA] receptor subunit epsilon-2 antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
      • Glutamate receptor ionotropic NMDA2B antibody
      • Glutamate receptor subunit epsilon 2 antibody
      • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
      • GRIN 2B antibody
      • GRIN2B antibody
      • hNR 3 antibody
      • hNR3 antibody
      • MGC142178 antibody
      • MGC142180 antibody
      • MRD6 antibody
      • N methyl D asparate receptor channel subunit epsilon 2 antibody
      • N methyl D aspartate receptor subtype 2B antibody
      • N methyl D aspartate receptor subunit 2B antibody
      • N methyl D aspartate receptor subunit 3 antibody
      • N-methyl D-aspartate receptor subtype 2B antibody
      • N-methyl-D-aspartate receptor subunit 3 antibody
      • NMDA NR2B antibody
      • NMDA R2B antibody
      • NMDAR2B antibody
      • NMDE2 antibody
      • NMDE2_HUMAN antibody
      • NME2 antibody
      • NR2B antibody
      • NR3 antibody
      see all

    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] 图像

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : mouse brain lysates (untreated)
      Lane 2 : mouse brain lysates (treated with AP)

      Lysates/proteins at 10 µg per lane.

      Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 166 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)

    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)参考文献

    This product has been referenced in:
    • Raval AP  et al. Chronic nicotine exposure inhibits estrogen-mediated synaptic functions in hippocampus of female rats. Neurosci Lett 517:41-6 (2012). WB ; Rat . Read more (PubMed: 22521583) »
    • Skelding KA  et al. aCaMKII is differentially regulated in brain regions that exhibit differing sensitivities to ischemia and excitotoxicity. J Cereb Blood Flow Metab : (2012). Read more (PubMed: 22929440) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Cell lysate - whole cell (brain)
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 8%)
    Loading amount 15 µg
    Treatment brain
    Specification brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

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    提交于 Apr 21 2017