概述

  • 产品名称Anti-NMDAR2B抗体[S59-36]
    参阅全部 NMDAR2B 一抗
  • 描述
    小鼠单克隆抗体[S59-36] to NMDAR2B
  • 特异性No cross-reactivity with NMDAR2A.
  • 经测试应用适用于: Flow Cyt, ICC/IF, WB, IP, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to extracellular N terminal amino acids 20 - 271 of Rat NMDAR2B (Acc. No. Q00960).

  • 阳性对照
    • WB: rat brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab93610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 - 1 µg/ml. Antigen retrieval is not essential but may optimise staining.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.

靶标

  • 功能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • 组织特异性Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all

Anti-NMDAR2B antibody [S59-36] 图像

  • Overlay histogram showing SH-SY5Y cells stained with ab93610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93610, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback

Anti-NMDAR2B antibody [S59-36] (ab93610)参考文献

This product has been referenced in:
  • Li C  et al. The extremely low-frequency magnetic field exposure differently affects the AMPAR and NMDAR subunit expressions in the hippocampus, entorhinal cortex and prefrontal cortex without effects on the rat spatial learning and memory. Environ Res 134C:74-80 (2014). Read more (PubMed: 25046815) »

See 1 Publication for this product

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