Anti-NMDAR2B抗体(ab73001)
Key features and details
- Rabbit polyclonal to NMDAR2B
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-NMDAR2B抗体
参阅全部 NMDAR2B 一抗 -
描述
兔多克隆抗体to NMDAR2B -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Chicken, Cow, Dog, Human, Non human primates -
免疫原
Synthetic peptide from the N-terminus of rat NMDAR2B.
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阳性对照
- Rat hippocampal lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73001于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
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ICC/IF |
1/500.
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说明 |
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WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa). |
ICC/IF
1/500. |
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
组织特异性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 417965 Chicken
- Entrez Gene: 537804 Cow
- Entrez Gene: 494009 Dog
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q5R1P3 Dog
see all -
别名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
图片
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Anti-NMDAR2B antibody (ab73001) at 1/1000 dilution + rat hippocampal lysate at 10 µg
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: <68 kDa, 120 kDa. We are unsure as to the identity of these extra bands. -
ab73001 diluted 1/500 staining NMDAR2B on 14DIV cultured rat cortical neurons fixed in methanol at - 20oC for 10 minutes. Sections were also labelled with an anti-PSD95 antibody in red.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab73001 被引用在 3 文献中.
- Song Y et al. A novel mechanism of synaptic and cognitive impairments mediated via microRNA-30b in Alzheimer's disease. EBioMedicine N/A:N/A (2018). PubMed: 30522932
- Zhang J & Chen C Alleviation of Neuropathology by Inhibition of Monoacylglycerol Lipase in APP Transgenic Mice Lacking CB2 Receptors. Mol Neurobiol 55:4802-4810 (2018). PubMed: 28733897
- Zhang J et al. Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease. J Neurosci 34:14919-33 (2014). WB ; Mouse . PubMed: 25378159