Anti-NMDAR2A (phospho Y1325)抗体(ab106590)

概述

  • 产品名称Anti-NMDAR2A (phospho Y1325)抗体
    参阅全部 NMDAR2A 一抗
  • 描述
    兔多克隆抗体to NMDAR2A (phospho Y1325)
  • 特异性Specific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Dog
  • 免疫原

    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDAR2A.

  • 阳性对照
    • Rat hippocampal lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 165 kDa.

靶标

  • 功能NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Anti-NMDAR2A (phospho Y1325) antibody 图像

  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution

    Lane 1 : Rat hippocampal lysate
    Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide


    Predicted band size : 165 kDa
    Observed band size : ~180 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution

    Lane 1 : Rat brain whole tissue lysate
    Lane 2 : Rat brain whole tissue lysate
    Lane 3 : Rat brain whole tissue lysate
    Lane 4 : Rat brain whole tissue lysate
    Lane 5 : Rat brain whole tissue lysate
    Lane 6 : Rat brain whole tissue lysate
    Lane 7 : Rat brain whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 165 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Xiaofan Wang

    See Abreview

Anti-NMDAR2A (phospho Y1325) antibody (ab106590)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing (3-8% Acetate)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Xiaofan Wang

Verified customer

提交于 Apr 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"