RabMAb

Anti-NMDAR1 (phospho S889)抗体[EPR2480Y] (ab68144)

概述

  • 产品名称Anti-NMDAR1 (phospho S889)抗体[EPR2480Y]
    参阅全部 NMDAR1 一抗
  • 描述
    兔单克隆抗体[EPR2480Y] to NMDAR1 (phospho S889)
  • 特异性This antibody detects NMDAR1 phosphorylated at serine 889.
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide corresponding to residues surrounding serine 889 of human NMDAR1

  • 阳性对照
    • Mouse brain lysate; human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab68144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).Can be blocked with NMDAR1 peptide (ab168728).
IP 1/30.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
    • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
    • 翻译后修饰NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
    • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    Anti-NMDAR1 (phospho S889) antibody [EPR2480Y] 图像

    • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68144 at a dilution of 1/100 - 1/250.
    • All lanes : Anti-NMDAR1 (phospho S889) antibody [EPR2480Y] (ab68144) at 1/2000 dilution

      Lane 1 : mouse brain lysate, cells untreated
      Lane 2 : mouse brain lysate, cells treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 105 kDa
      Observed band size : 105 kDa

    Anti-NMDAR1 (phospho S889) antibody [EPR2480Y] (ab68144)参考文献

    This product has been referenced in:
    • D'Aiuto L  et al. Human induced pluripotent stem cell-derived models to investigate human cytomegalovirus infection in neural cells. PLoS One 7:e49700 (2012). WB . Read more (PubMed: 23209593) »

    See 1 Publication for this product

    Product Wall

    The peptide datasheet is at this link: http://www.abcam.com/index.html?datasheet=168728.

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
    Would you be so kind to check the batch number (GR257227-5) again since our system does not recognzie it.
    After reading through t...

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    Gracias por ponerse en contacto con nosotros. Dependiendo de la técnica que vaya a utilizar, le puede convenir mas usar un tipo de anticuerpos u otro. Ya que los anticuerpos policlonales son capaces de reconocer múltiples epítopos, éstos presentan ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"