Anti-NMDAR1抗体- Neuronal Marker (ab17345)

概述

  • 产品名称Anti-NMDAR1抗体- Neuronal Marker
    参阅全部 NMDAR1 一抗
  • 描述
    兔多克隆抗体to NMDAR1 - Neuronal Marker
  • 经测试应用适用于: IHC (PFA fixed), WB, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat NMDAR1.

    (Peptide available as ab17568.)

  • 阳性对照
    • Rat brain and spinal cord

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab17345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC (PFA fixed) Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).Can be blocked with Rat NMDAR1 peptide (ab17568).
IHC-FoFr Use at an assay dependent concentration. PubMed: 20831598
ICC/IF 1/1000.

靶标

  • 功能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • 翻译后修饰NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

Anti-NMDAR1 antibody - Neuronal Marker 图像

  • ab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5µg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.

  • Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml + HEK293 cell lysate co-transfected with NR1 and NR2A recombinant protein at 50 µg

    Predicted band size : 105 kDa
    Observed band size : ~110 kDa (why is the actual band size different from the predicted?)

    HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa).  ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2µg/ml.

  • All lanes : Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml

    Lane 1 : HEK293 Whole Cell Lysate Transfected with SHAM
    Lane 2 : HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    NMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown).

Anti-NMDAR1 antibody - Neuronal Marker (ab17345)参考文献

This product has been referenced in:
  • Bach EC  et al. Enhanced NMDA receptor-mediated modulation of excitatory neurotransmission in the dorsal vagal complex of streptozotocin-treated, chronically hyperglycemic mice. PLoS One 10:e0121022 (2015). Read more (PubMed: 25799386) »
  • Cahill E  et al. D1R/GluN1 complexes in the striatum integrate dopamine and glutamate signalling to control synaptic plasticity and cocaine-induced responses. Mol Psychiatry N/A:N/A (2014). Read more (PubMed: 25070539) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

We have a few rabbit-raised antibodies to NMDAR1 that were tested in mouse as well as WB and ICC.

ab28669 (http://www.abcam.com/NMDAR1-antibody-ab28669.html), but based on the immunogen sequence, it wou...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary Neurons Cortex)
Specification Primary Neurons Cortex
Fixative Formaldehyde
Permeabilization Yes - Methanol
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Apr 11 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 07 2007

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (hippocampus and cortex)
Specification hippocampus and cortex
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Apr 12 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"