RabMAb

Anti-NMDAR1抗体[EPR2481(2)] (ab109182)

概述

  • 产品名称Anti-NMDAR1抗体[EPR2481(2)]
    参阅全部 NMDAR1 一抗
  • 描述
    兔单克隆抗体[EPR2481(2)] to NMDAR1
  • 经测试应用适用于: WBmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NMDAR1 aa 850-950.

  • 阳性对照
    • Fetal brain cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

性能

应用

Our Abpromise guarantee covers the use of ab109182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
    • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
    • 翻译后修饰NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
    • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    Anti-NMDAR1 antibody [EPR2481(2)] 图像

    • All lanes : Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 105 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 105 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 105 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (unpurified) + Human fetal brain cell lysate at 10 µg

      Predicted band size : 105 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

      Secondary antibody - anti-rabbit HRP (ab6721)

    Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)参考文献

    This product has been referenced in:
    • Zhang X  et al. A lasting effect of postnatal sevoflurane anesthesia on the composition of NMDA receptor subunits in rat prefrontal cortex. Int J Dev Neurosci 54:62-69 (2016). WB ; Rat . Read more (PubMed: 27025552) »
    • Ho YC  et al. Hypofunction of glutamatergic neurotransmission in the periaqueductal gray contributes to nerve-injury-induced neuropathic pain. J Neurosci 33:7825-36 (2013). Read more (PubMed: 23637174) »

    See all 2 Publications for this product

    Product Wall

    That is correct, according to Uniprot the exact immunogen sequence is missing in isoforms 1, 2 and 7 but conserved in isoforms 3 (also known as long, NR-1-3), 4, 5 and 6.

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
    Sample Rat Tissue lysate - other (hippocampi total membrane fraction)
    Specification hippocampi total membrane fraction
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Ms. Sunetra Jitkar

    Verified customer

    提交于 Feb 19 2014

    The protocol used was our standard FC protocol with ab109182 Anti-NMDAR1 antibody [EPR2481(2)]. Please see the link below for more details.

    http://www.epitomics.com/products/support/fc

    Thank you for your enquiry.

    Unfortunately, we do not have the epitope information for this antibody but according to the immunogen sequence, it is towards the cytoplasmic region of the membrane. Therefore, this product may not be suitable fo...

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    Thank you for your enquiry.

    This product was tested with permeabilized SH‐SY5Y cells and the cells were fixed with 2% paraformaldehyde and incubated at room temperature for 10 min. Our labs use the intracellular staining protocol only, and s...

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    Thank you for contacting us.

    We have a few rabbit-raised antibodies to NMDAR1 that were tested in mouse as well as WB and ICC.

    ab28669 (http://www.abcam.com/NMDAR1-antibody-ab28669.html), but based on the immunogen sequence, it wou...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"