概述

  • 产品名称
  • 描述
    山羊多克隆抗体to NMDAR1
  • 特异性
    This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chicken, Dog, Human, Xenopus laevis, Duck, Zebrafish
  • 免疫原

    Synthetic peptide:

    TQERVNNSNKKE

    , corresponding to internal sequence amino acids 486-497 from the internal region of Human NMDAR1 according to NP_000823.4; NP_067544.1; NP_015566.1

  • 阳性对照
    • Rat brain lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:1,000.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 and 105 kDa (predicted molecular weight: 105 kDa).

    The 23kDa band was strong in mouse brain lysates and faint in human brain lysates. The band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
    • 序列相似性
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
    • 翻译后修饰
      NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
    • 细胞定位
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    Anti-NMDAR1 antibody 图像

    • Anti-NMDAR1 antibody (ab77264) at 2 µg/ml + rat brain lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 105 kDa
      Observed band size : 105 kDa
      Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    Anti-NMDAR1 antibody (ab77264)参考文献

    This product has been referenced in:
    • Cronican AA  et al. Genome-wide alteration of histone H3K9 acetylation pattern in mouse offspring prenatally exposed to arsenic. PLoS One 8:e53478 (2013). WB ; Mouse . Read more (PubMed: 23405071) »
    • Ghafari M  et al. NMDA-complexes linked to spatial memory performance in the Barnes maze in CD1 mice. Behav Brain Res : (2011). WB ; Mouse . Read more (PubMed: 21377497) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Chicken Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Loading amount
    40 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 23 2017

    Thank you for your message and for your patience.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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