概述

  • 产品名称Anti-NMDAR1抗体
    参阅全部 NMDAR1 一抗
  • 描述
    兔多克隆抗体to NMDAR1
  • 特异性ab52177 detects endogenous levels of total NMDAR1 protein.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human NMDAR1 around the phosphorylation site of serine 897 (R-S-S-K-D).

  • 阳性对照
    • Human brain tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab52177 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 105 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • 翻译后修饰NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

Anti-NMDAR1 antibody 图像

  • ab52177 at 1/50 dilution staining NMDAR1 in human brain tissue by immunohistochemistry, paraffin embedded tissue in the presence (right image) or absence (left image) of the immunisng peptide.
  • All lanes : Anti-NMDAR1 antibody (ab52177) at 1/500 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 105 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Anti-NMDAR1 antibody (ab52177)参考文献

This product has been referenced in:
  • Roshanravan H  et al. NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801. Diabetes 65:3139-50 (2016). Read more (PubMed: 27388219) »
  • Sun H  et al. Involvement of NR1, NR2A different expression in brain regions in anxiety-like behavior of prenatally stressed offspring. Behav Brain Res 257:1-7 (2013). Read more (PubMed: 24029697) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Zebrafish Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Narin ılgım Ardıç

Verified customer

提交于 May 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Permeabilization No
Fixative Paraformaldehyde
Username

Mrs. Lola Sanchez

Verified customer

提交于 Feb 26 2015

Thanks for your email.

I have located your order. I am happy to send a vial of anti-GFAP antibody ab53554 as the replacement. We do have this product in stock and I will ship it Monday for Tuesday delivery. I will send you an email containing...

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Thanks for your email.

I did see the western blot image that you kindly attached to your last email. There are a few bands found in the "No Primary Ab" blot but not nearly as many as can be seen in the blot on the left which include...

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Thanks for your email.

I am sorry this antibody is giving you trouble. I am happy to offer a replacement of any primary antibody, credit or refund. If you are interested, please let me know how you wish to proceed. In your reply, kindly inclu...

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Thanks for your reply.

For a negative control, I was thinking of samples in which you do not expect to see NMDAR1 expression. Since there is more than one bandinyour sample lanesI think it would be helpful to compare a sample where there is d...

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Thanks so much for all your troubleshooting efforts!

Do the gels from your colleague your the gel image you sent on Sunday contain any negative controls samples, ie. samples in which you do not expect to see NMDAR1 expression? If so, please n...

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Thanks for your enquiry and for including the western blot image.

It does not appear that NMDAR1 is being detectedon your western blot. I just have a couple additional questions:

1. How much protein was loaded per lane on the wester...

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