概述

  • 产品名称
    Anti-NMDAR1抗体[2C6.2]
    参阅全部 NMDAR1 一抗
  • 描述
    小鼠单克隆抗体[2C6.2] to NMDAR1
  • 特异性
    ab64572 recognises the C2 cassette region at the C terminal end of NMDAR1. The clone number has been updated from (2Q155) to (2C6.2) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: WB, IHC (PFA fixed), IHC-Glutmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide:

    LQNQKDTVLPRRAIEREEGQLQLCSRHRES

    , corresponding to C terminal amino acids 909-938 of Rat NMDAR1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 40% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 250mM Sodium chloride, 20mM PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    2C6.2
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 105 kDa.
IHC (PFA fixed) Use at an assay dependent concentration.
IHC-Glut 1/100 - 1/500. An antigen retrieval step is recommended.

靶标

  • 功能
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • 序列相似性
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • 翻译后修饰
    NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • 细胞定位
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

文献

This product has been referenced in:
  • McGee MA & Abdel-Rahman AA Enhanced vascular neuronal nitric-oxide synthase-derived nitric-oxide production underlies the pressor response caused by peripheral N-methyl-D-aspartate receptor activation in conscious rats. J Pharmacol Exp Ther 342:461-71 (2012). WB ; Rat . Read more (PubMed: 22580349) »

See 1 Publication for this product

客户评价及客户问答

You're correct! We're sending you 125ug of ab64572 at 1 mg/ml so you're going to receive 125 ul.

You could store it at -20C without glycerol, however adding the glycerol will protect it from freezing at -20C so our recommendation would be to...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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