概述

  • 产品名称
    NMDA
  • 描述
    Excitotoxic amino acid
  • 生物学描述
    Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy. Also available in Kit: Ionotropic agonists (ab120323).

    Also available in simple stock solutions (ab146698) - add 1 ml of water to get an exact, ready-to-use concentration.
  • 纯度
    > 99%

性能

应用

Our Abpromise guarantee covers the use of ab120052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

文献

This product has been referenced in:
  • Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »
  • Collingridge GL & Lester RA Excitatory amino acid receptors in the vertebrate central nervous system. Pharmacol Rev 41:143-210 (1989). Read more (PubMed: 2558391) »
  • Hansen JJ & Krogsgaard-Larsen P Structural, conformational, and stereochemical requirements of central excitatory amino acid receptors. Med Res Rev 10:55-94 (0). Read more (PubMed: 2153264) »

See all 12 Publications for this product

客户评价及客户问答

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I am sorry to hear you are not observing the expected results with the reagent supplied.
It is possible that ab120052 acts more potently due to it having a higher purity when compared to Sigma’s. I have attached t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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