Anti-NM23A抗体[EPR3036] (ab92327)


  • 产品名称Anti-NM23A抗体[EPR3036]
    参阅全部 NM23A 一抗
  • 描述
    兔单克隆抗体[EPR3036] to NM23A
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human NM23A.

  • 阳性对照
    • WB: MCF7, A375, HeLa and Jurkat cell lysates IHC-P: Human lung adenocarcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab92327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Overnight incubation is recommended.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
    • 组织特异性Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.
    • 序列相似性Belongs to the NDK family.
    • 细胞定位Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AWD antibody
      • AWD, drosophila, homolog of antibody
      • GAAD antibody
      • Granzyme A activated DNase antibody
      • Granzyme A-activated DNase antibody
      • GZMA activated DNase antibody
      • Metastasis inhibition factor NM23 antibody
      • NB antibody
      • NBS antibody
      • NDK A antibody
      • NDKA antibody
      • NDKA_HUMAN antibody
      • NDP kinase A antibody
      • NDPK-A antibody
      • NDPKA antibody
      • NM23 antibody
      • NM23 long variant, included antibody
      • nm23-H1 antibody
      • NM23-M1 antibody
      • NM23H1B, included antibody
      • NME/NM23 nucleoside diphosphate kinase 1 antibody
      • Nme1 antibody
      • NME1-NME2 spliced read-through transcript, included antibody
      • Non-metastatic cells 1, protein (NM23A) expressed in antibody
      • Nonmetastatic cells 1, protein expressed in antibody
      • Nonmetastatic protein 23 antibody
      • Nonmetastatic protein 23, homolog 1 antibody
      • Nucleoside diphosphate kinase A antibody
      • Tumor metastatic process-associated protein antibody
      see all

    Anti-NM23A antibody [EPR3036] 图像

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NM23A with unpurified ab92327 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/5000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 17 kDa
    • ab92327, at a dilution of 1/100, staining NM23A in formalin fixed, paraffin embedded Human lung adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining

    Anti-NM23A antibody [EPR3036] (ab92327)参考文献

    ab92327 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92327.
    Please use the links above to contact us or submit feedback about this product.