概述

  • 产品名称
  • 描述
    兔多克隆抗体to NLRP3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 974 and 1036 of Human CIAS1/ NALP3 (NP_001073289).

  • 阳性对照
    • Raji cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab98151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 118 kDa.

靶标

  • 功能
    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • 组织特异性
    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • 疾病相关
    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • 序列相似性
    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGTAVPRL antibody
    • AII/AVP antibody
    • Angiotensin/vasopressin receptor AII/AVP like antibody
    • Angiotensin/vasopressin receptor AII/AVP-like antibody
    • C1orf7 antibody
    • Caterpiller protein 1.1 antibody
    • CIAS 1 antibody
    • CIAS1 antibody
    • CLR1.1 antibody
    • Cold autoinflammatory syndrome 1 antibody
    • Cold autoinflammatory syndrome 1 protein antibody
    • Cryopyrin antibody
    • Familial cold autoinflammatory syndrome antibody
    • FCAS antibody
    • FCU antibody
    • LRR and PYD domains-containing protein 3 antibody
    • Muckle-Wells syndrome antibody
    • MWS antibody
    • NACHT antibody
    • NACHT LRR and PYD containing protein 3 antibody
    • NALP 3 antibody
    • NALP3 antibody
    • NALP3_HUMAN antibody
    • NLR family pyrin domain containing 3 antibody
    • NLRP3 antibody
    • PYPAF 1 antibody
    • PYPAF1 antibody
    • PYRIN containing APAF1 like protein 1 antibody
    • PYRIN-containing APAF1-like protein 1 antibody
    see all

Anti-NLRP3 antibody 图像

  • Anti-NLRP3 antibody (ab98151) at 1/1500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 118 kDa
    7.5% SDS PAGE

实验方案

Anti-NLRP3 antibody (ab98151)参考文献

This product has been referenced in:
  • Cao S  et al. Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation. Sci Rep 7:2417 (2017). WB . Read more (PubMed: 28546552) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (LX-2 Human Hepatic Stellate Cell Line)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
LX-2 Human Hepatic Stellate Cell Line
Username

Abcam user community

Verified customer

提交于 Jul 10 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (LX-2 Human Hepatic Stellate Cell Line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
LX-2 Human Hepatic Stellate Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册