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No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field.
Our Abpromise guarantee covers the use of ab4207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
ab4207 staining CIAS1 / NALP3 in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab4207 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor® 488 conjugated donkey anti-goat (H+L) polyclonal used at a 1/500 dilution.WGA: Alexa Fluor® 568 Wheat Germ Agglutinin.
Immunofluorescence analysis of Human MDM cells, staining CIAS1 / NALP3 with ab4207.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% goat serum for 1 hour at room temp. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 12 hours at 4°C. A Cy5® conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.
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