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概述

  • 产品名称
    Anti-NLRC3抗体
    参阅全部 NLRC3 一抗
  • 描述
    兔多克隆抗体to NLRC3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human NLRC3.

    参阅Abcam的专有抗源政策

    (Peptide available as ab90252.)

  • 阳性对照
    • This antibody gave a positive signal in human lymph node tissue lysate and the following whole cell lysates: Raji; Ramos; Jurkat.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).

靶标

  • 功能
    May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA.
  • 组织特异性
    Detected in peripheral blood mononuclear cells.
  • 序列相似性
    Belongs to the NLRP family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CARD15-like antibody
      • CARD15-like protein antibody
      • Caterpiller 16.2 antibody
      • Caterpiller protein 16.2 antibody
      • CLR16.2 antibody
      • FLJ00348 antibody
      • NLR family CARD domain containing 3 antibody
      • Nlrc3 antibody
      • NLRC3_HUMAN antibody
      • NOD-like receptor C3 antibody
      • NOD3 antibody
      • NOD3 protein antibody
      • Nucleotide-binding oligomerization domain protein 3 antibody
      • Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 antibody
      • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 antibody
      • Protein NLRC3 antibody
      see all

    Anti-NLRC3 antibody 图像

    • Western blot - NLRC3 antibody (ab77817)
      Western blot - NLRC3 antibody (ab77817)
      All lanes : Anti-NLRC3 antibody (ab77817) at 1 µg/ml

      Lane 1 : Human lymph node tissue lysate - total protein (ab29871)
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 114 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 33 kDa,70 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 5 minutes

    实验方案

    Anti-NLRC3 antibody (ab77817)参考文献

    ab77817 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab77817？请让我们知道，以便我们可以引用本数据表中的参考文章。

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