概述

  • 产品名称
    Anti-NLRC3抗体
    参阅全部 NLRC3 一抗
  • 描述
    兔多克隆抗体to NLRC3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human NLRC3.

    参阅Abcam的专有抗源政策

    (Peptide available as ab90252.)

  • 阳性对照
    • This antibody gave a positive signal in human lymph node tissue lysate and the following whole cell lysates: Raji; Ramos; Jurkat.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).

靶标

  • 功能
    May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA.
  • 组织特异性
    Detected in peripheral blood mononuclear cells.
  • 序列相似性
    Belongs to the NLRP family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CARD15-like antibody
      • CARD15-like protein antibody
      • Caterpiller 16.2 antibody
      • Caterpiller protein 16.2 antibody
      • CLR16.2 antibody
      • FLJ00348 antibody
      • NLR family CARD domain containing 3 antibody
      • Nlrc3 antibody
      • NLRC3_HUMAN antibody
      • NOD-like receptor C3 antibody
      • NOD3 antibody
      • NOD3 protein antibody
      • Nucleotide-binding oligomerization domain protein 3 antibody
      • Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 antibody
      • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 antibody
      • Protein NLRC3 antibody
      see all

    图片

    • Western blot - NLRC3 antibody (ab77817)
      Western blot - NLRC3 antibody (ab77817)
      All lanes : Anti-NLRC3 antibody (ab77817) at 1 µg/ml

      Lane 1 : Human lymph node tissue lysate - total protein (ab29871)
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 114 kDa
      Observed band size: 105 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 33 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 5 minutes


      NLRC3 has four possible isoforms which have the following predicted molecular weights, 114, 119, 83 and 111 kDa (SwissProt data).

    实验方案

    文献

    ab77817 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab77817？请让我们知道，以便我们可以引用本数据表中的参考文章。

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