Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human NLRC3.
(Peptide available as ab90252.)
- This antibody gave a positive signal in human lymph node tissue lysate and the following whole cell lysates: Raji; Ramos; Jurkat.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).
May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA.
Detected in peripheral blood mononuclear cells.
Belongs to the NLRP family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
Information by UniProt
- CARD15-like antibody
- CARD15-like protein antibody
- Caterpiller 16.2 antibody
Western blot - NLRC3 antibody (ab77817)
All lanes :
Anti-NLRC3 antibody (ab77817) at 1 µg/mlLane 1 :
Human lymph node tissue lysate - total protein (ab29871
)Lane 2 :
Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 :
Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 4 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
114 kDaObserved band size:
105 kDa (why is the actual band size different from the predicted?
)Additional bands at:
33 kDa, 70 kDa. We are unsure as to the identity of these extra bands.Exposure time:
NLRC3 has four possible isoforms which have the following predicted molecular weights, 114, 119, 83 and 111 kDa (SwissProt data).
has not yet been referenced specifically in any publications.
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