概述

  • 产品名称Anti-NLN抗体[1D6]
  • 描述
    小鼠单克隆抗体[1D6] to NLN
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    Recombinant full length Human NLN produced in HEK293T cells (NP_065777).

  • 阳性对照
    • HEK293T cells transfected with NLN, HepG2, HeLa, SVT2, A549, COS7, PC12, and MCF7 cell extracts, Carcinoma of Human pancreas, Adenocarcinoma of Human endometrium, Carcinoma of Human bladder and Human tonsil tissues, COS7 cells transiently transfected with NLN
  • 常规说明Dilute in PBS (pH7.3) before use.

性能

应用

Our Abpromise guarantee covers the use of ab119802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 81 kDa.
IHC-P 1/150.
ICC/IF 1/100.

靶标

Anti-NLN antibody [1D6] 图像

  • All lanes : Anti-NLN antibody [1D6] (ab119802) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NLN cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 81 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC212447)
  • All lanes : Anti-NLN antibody [1D6] (ab119802) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 81 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC212447)
  • ab119802 at 1/150 dilution staining NLN in Paraffin-embedded Human Pancreas carcioma tissue by Immunohistochemistry.
  • ab119802 at 1/150 dilution staining NLN in Paraffin-embedded Human Endometrium adenocarcinoma carcioma tissue by Immunohistochemistry.
  • ab119802 at 1/150 dilution staining NLN in Paraffin-embedded Human Bladder carcioma tissue by Immunohistochemistry.
  • ab119802 at 1/150 dilution staining NLN in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • ab119802 at 1/100 dilution staining NLN in COS7 cells transiently transfected with pCMV6-ENTRY NLN by Immunofluorescence.

Anti-NLN antibody [1D6] (ab119802)参考文献

ab119802 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"