概述

  • 产品名称Anti-NLK抗体
    参阅全部 NLK 一抗
  • 描述
    兔多克隆抗体to NLK
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Chicken, Dog, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human NLK.

    (Peptide available as ab26986.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Mouse Lung Normal; Formalin Fixed Paraffin Embedded Mouse Brain Normal. This antibody gave a positive signal in the following cell types: U-87 MG.

性能

应用

Our Abpromise guarantee covers the use of ab26050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme.
  • 细胞定位Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp761G1211 antibody
    • FLJ21033 antibody
    • LAK1 antibody
    • Nemo like kinase antibody
    • Nemo-like kinase antibody
    • Nlk antibody
    • NLK_HUMAN antibody
    • Protein LAK1 antibody
    • Serine/threonine protein kinase NLK antibody
    • Serine/threonine-protein kinase NLK antibody
    see all

Anti-NLK antibody 图像

  • Anti-NLK was used in standard Vector ABC protocol on paraffin embedded mouse lung with the following modifications: HIER with Vector antigen unmasking solution for 25 minutes at 98C. Used a 1:100 dilution in PBS/Triton-X 4C overnight.
  • Adult mouse brain fixed in 10% neutral buffered formulin. Antigen retrieval was performed using Tris-EDTA pH 9.0. The tissue was incubated with ab26050 at a concentration of 2.5µg/ml for 16 hours at 4°C. Nuclear staining can be clearly seen. 

  • ICC/IF image of ab26050 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26050 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NLK antibody (ab26050)参考文献

This product has been referenced in:
  • Rydell-Törmänen K  et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . Read more (PubMed: 26997628) »
  • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Kidney cancer cell)
Loading amount 20 µg
Specification Kidney cancer cell
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Nov 16 2010

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Human prostate cancer)
Loading amount 50 µg
Specification Human prostate cancer
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mrs. Tiffany Pitts

Verified customer

提交于 May 31 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"