Synthetic peptide derived from an internal sequence of human NLE1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 53 kDa).
Belongs to the NLE1/RSA4 family.
Contains 8 WD repeats.
Nucleus > nucleolus.
Information by UniProt
- AL022765 antibody
- BC018399 antibody
- FLJ10458 antibody
Western blot - NLE1 antibody (ab74143)
All lanes :
Anti-NLE1 antibody (ab74143) at 1/500 dilutionLane 1 :
Extracts from COS cellsLane 2 :
Extracts from COS cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.Predicted band size :
53 kDaObserved band size :
48 kDa (why is the actual band size different from the predicted?
)Additional bands at :
35 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"