Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human nkx6.3.
This antibody gave a positive signal in the following lysates:
E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 mg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Putative transcription factor, which may be involved in patterning of central nervous system and pancreas.
Contains 1 homeobox DNA-binding domain.
Information by UniProt
Homeobox protein Nkx 6.3 antibody
Homeobox protein Nkx-6.3 antibody
Western blot - Anti-nkx6.3 antibody (ab93235)
Anti-nkx6.3 antibody (ab93235) at 1 µg/ml + E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate at 10 µg
Secondary Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa Additional bands at: 38 kDa, 42 kDa, 90 kDa. We are unsure as to the identity of these extra bands. Exposure time: 12 minutes
Immunocytochemistry/ Immunofluorescence - Anti-nkx6.3 antibody (ab93235)
ICC/IF image of ab93235 stained SNKSH cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93235, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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