Anti-nkx6.3抗体(ab69946)

概述

  • 产品名称Anti-nkx6.3抗体
    参阅全部 nkx6.3 一抗
  • 描述
    兔多克隆抗体to nkx6.3
  • 特异性ab69946 detects endogenous levels of total nkx6.3 protein.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human nkx6.3

  • 阳性对照
    • HUVEC cells and cell extracts from K562 and COS7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab69946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

Anti-nkx6.3 antibody 图像

  • ab69946 at 1/500 dilution staining nkx6.3 in HUVEC cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-nkx6.3 antibody (ab69946) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : COS7 cell extract
    Lane 3 : K562 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

Anti-nkx6.3 antibody (ab69946)参考文献

ab69946 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step 0.3M glycine, donkey serum, 1% BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Mouse Cell (adult stomach, embryonic brain)
Specification adult stomach, embryonic brain
Permeabilization Yes - 0.5% Triton-X 100
Fixative Paraformaldehyde
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提交于 Sep 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"