Anti-nkx6.2抗体(ab93189)

概述

  • 产品名称Anti-nkx6.2抗体
    参阅全部 nkx6.2 一抗
  • 描述
    兔多克隆抗体to nkx6.2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human nkx6.2.

  • 阳性对照
    • This antibody gave a positive signal in Human Brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab93189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

靶标

Anti-nkx6.2 antibody 图像

  • Anti-nkx6.2 antibody (ab93189) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 46 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-nkx6.2 antibody (ab93189)参考文献

ab93189 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic spinal cord)
Permeabilization Yes - 0.1%Triton X-100
Specification Embryonic spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Dr. Frederic Clotman

Verified customer

提交于 Jun 10 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"