Anti-Nkx2.5抗体(ab106923)

概述

  • 产品名称Anti-Nkx2.5抗体
    参阅全部 Nkx2.5 一抗
  • 描述
    山羊多克隆抗体to Nkx2.5
  • 特异性ab106923 is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-PRAYSDPDPAKDPR

    , corresponding to internal sequence amino acids 94-107 of Human Nkx2.5 (NP_004378.1; NP_001159647.1; NP_001159648.1).

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Human fetal heart; Mouse heart; Rat Heart
  • 常规说明


性能

应用

Our Abpromise guarantee covers the use of ab106923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).

Abcam recommends using 3% milk as the blocking agent.

靶标

  • 功能Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • 组织特异性Expressed only in the heart.
  • 疾病相关Atrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • 序列相似性Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NKX2.5, mouse, homolog of antibody
    • Cardiac-specific homeobox 1 antibody
    • Cardiac-specific homeobox antibody
    • CHNG5 antibody
    • CSX antibody
    • CSX1 antibody
    • FLJ52202 antibody
    • FLJ97166 antibody
    • FLJ97195 antibody
    • FLJ97197 antibody
    • FLJ99536 antibody
    • HLHS2 antibody
    • Homeobox protein CSX antibody
    • Homeobox protein NK-2 homolog E antibody
    • Homeobox protein Nkx 2.5 antibody
    • Homeobox protein Nkx-2.5 antibody
    • NK2 homeobox 5 antibody
    • NK2 transcription factor related locus 5 antibody
    • NK2 transcription factor related, locus 5 (Drosophila) antibody
    • NK2, Drosophila, homolog of, E antibody
    • NKX2-5 antibody
    • NKX2.5 antibody
    • NKX25_HUMAN antibody
    • Nkx2E antibody
    • NKX4-1 antibody
    • Tinman paralog antibody
    • VSD3 antibody
    see all

Anti-Nkx2.5 antibody 图像

  • All lanes : Anti-Nkx2.5 antibody (ab106923) at 1 µg/ml (Blocked with 3% Milk)

    Lane 1 : Human Nkx2.5 recombinant protein (tagged) at 0.1 µg
    Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) at 20 µg
    Lane 3 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate (negative control) at 20 µg
    Lane 4 : Heart (Mouse) Mouse Tissue Lysate at 20 µg
    Lane 5 : Heart (Rat) Tissue Lysate at 20 µg
    Lane 6 : Human Fetal Heart - Total Protein Tissue Lysate at 20 µg

    Secondary
    Rabbit Anti-Goat IgG H&L (HRP) (ab97100) at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106923 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Anti-Nkx2.5 antibody (ab106923) at 0.3 µg/ml + Human heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Primary incubation was 1 hour.

    The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).

Anti-Nkx2.5 antibody (ab106923)参考文献

ab106923 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (H9C2 cell line)
Loading amount 150000 cells
Specification H9C2 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Maria Angela d'Amico

Verified customer

提交于 Dec 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"