概述

  • 产品名称Anti-Nkx2.2抗体
    参阅全部 Nkx2.2 一抗
  • 描述
    兔多克隆抗体to Nkx2.2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (SLTNTKTGFS VKDILDLPDT NDEEGSVAEG PEEENEGPEP AKRAGPLGQG) of Human Nkx2.2 (NP_002500).

  • 阳性对照
    • Placenta lysate.

性能

应用

Our Abpromise guarantee covers the use of ab85675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Nkx2.2 antibody 图像



  • Predicted band size : 30 kDa
    Western blot analysis of Human fetal brain tissue lysate labeling Nkx2.2 with ab85675 at 1.0µg/ml.
  • Anti-Nkx2.2 antibody (ab85675) at 1 µg/ml (5% skim milk / PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-Nkx2.2 antibody (ab85675)参考文献

ab85675 has not yet been referenced specifically in any publications.

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